The actor, 27, was accused by an anonymous Twitter account with the handle @Victori66680029 of sexual assault at a 2013 party. Similar allegations of sexual misconduct were made against his co-stars Vanessa Morgan and KJ Apa on other anonymous Twitter pages.
One of the accounts later tweeted, "Do you see how easy it is to lie and you guys will believe it? Vanessa Morgan and Kj Apa didn't do jack s**t. You will believe anything."
Taking to the platform on his own account, Sprouse addressed the allegations, writing, "Earlier today myself and three other cast mates were falsely accused of sexual offence by anonymous accounts on twitter. I take these accusations very seriously, and will be working with the right teams to get to the root of it."
He continued, "False accusations do tremendous damage to victims of actual assault. Furthermore, I would never seek to silence anybody. I encourage that people look into the accusations themselves, as the events detailed were factually untrue."
Signing off, he noted that, "This seems to be the latest claim in a series of incidents seeking to baselessly cancel my cast mates and me."
Meanwhile, his co-star and ex-girlfriend Reinhart, 23, quote tweeted Sprouse's series of tweets to speak out, insisting, "I have always taken sexual assault allegations seriously. But it was proven that this account was created specifically to create false stories about me and my cast. I can't think of something more twisted than lying about sexual assault. It invalidates the men and women who are brave enough to come forward with the truth."
She added, "This kind of lie can ruin lives and careers- and I can call it a lie because the person who made the allegations already admitted that the stories were fabricated."
"We are looking into taking legal action. This kind of sick behaviour only harms true survivors. This was incredibly triggering for me and scary because I've always supported survivors and believed them. I hope and pray that this does not discourage real survivors from speaking out about their experiences."
Morgan, 28, retweeted Sprouse's posts on Sunday evening, while Apa, 23, has not tweeted since June 14.