The singer and actress was just one of the famous faces taking part in the event, titled Celebration for Change, which raised just over $760,000 (£583,000) for the presidential candidate's campaign.
While Streisand didn't perform during the evening's entertainment, she was responsible for delivering eight minutes of closing remarks, during which she seized the opportunity to blast the current POTUS.
"Let's face it, Trump is unfit, mentally and morally, to hold this distinguished office," she said. "A few weeks ago, Trump called out the troops against peaceful protesters. That is what cowards do when they are scared. Donald Trump has failed us. And we cannot survive four more years of his incompetence we're feeling right now. (Biden) will listen to people rather than greeting them with guns. All they want is justice. It's as simple as that."
Reminding viewers that Biden was part of Barack Obama's administration, which helped the U.S. out of the recession in 2008, she added, "He's the leader we need now."
Others who didn't hold back while blasting Trump included Kristin Chenoweth, who performed a satirical song which included lyrics mocking what the president thinks makes a person popular.
"I'll tell you what ties to wear. Maybe dye your hair. Everything that really counts to be popular. You're going to be popular. You see I'm adorable. A deplorable. Well Joe, you can be that too. Be like me, and let's turn all of those red states blue," she sang.