The new season of players all meet up in the gym and Ali welcomes them. She warns the players that in order to stay on the ranch, they have to spend as much time as possible in the place they are standing.
Ali calls Bob and Jillian to the floor and the players get really excited and start greeting them with hugs. But the joy is short lived as the scale is revealed and the players have to weigh in for the first time on the ranch. Each player is shocked when they see what the scale says.
Ali tells the players that Bob and Jillian are going home for the night and everyone else will be staying to participate in a challenge. They step outside and see a line of treadmills. Ali tells the players that tonight's challenge will determine which trainer they will work with. But there's a new twist - the players get to choose to work out with Bob AND Jillian, or with two ALL-NEW trainers.
Each team talks out their strategy. The four weeks of immunity is looking very tempting to a lot of them. Once the timer starts, one player from each team gets on the treadmill to start their 5K. When they get exhausted, they tag their partner in and take a breather.
The Yellow Team is the first to complete the challenge and Ali wants to know who they've chosen as their trainers. The Yellow Team decides to go with the unknown trainers in exchange for four weeks of immunity. The Grey Team follows suit and decides to work out with the unknown trainers, as well.
In the end, the Aqua, Green, Orange, Purple, Blue and Black Teams all decide to work out with Bob and Jillian during their Biggest Loser journey.
Bob and Jillian find out the teams' trainer choices. Both Bob and Jillian grow a little nervous because the smartest decision each player could have made would have been to choose the four-week immunity.
Jillian tells the players she's going to work on not screaming this season, but that promise is quickly broken as she keeps pushing the players. Each team can't believe just how far they're being pushed while working out. Bob declares the biggest girl on the ranch, Courtney, is also the biggest threat in the game.
Meanwhile, the rest of the teams are working out at the Malibu Fitness Ridge with the mystery trainers. The teams are running, boxing, and doing obstacle courses. The mystery trainers' main goal is to teach the players how to fight for what they want.
Now it's time to see how Bob and Jillian's teams have done at the weigh in. Each team is a bit nervous, but in the end, very satisfied with their weight-loss numbers.
The Orange Team falls below the yellow line and the teams have vote on which woman to send home. In the end, the teams decide to send Ana home and keep her daughter Irene on the ranch.