The drama's creator, Jill Soloway, has confirmed Maura Pfefferman will not survive the fifth season's two-hour final show.
Tambor was fired from the Emmy-winning Amazon series following allegations of sexual misconduct. The actor has denied the allegations.
"When the time came to bring the "Transparent" journey to a close, it was clear that saying goodbye to Maura was our path forward," Soloway says in a statement.
The show will end with a musical, which will "dramatize the death of Maura in an odyssey of comedy and melancholy told through the joyful prism of melody and dance."
The date of the finale has not been announced, but "Transparent" is expected to return to TV later this year.