The "Fuller House" star, who has become a frequent backing player for the surf rockers, was onstage with the group in Dallas, Texas on Wednesday, March 20, when the back seam of his designer trousers came apart.
Stamos took to Twitter on Thursday to share photos of the wardrobe malfunction, which he jokingly blamed on pop rival Nick Jonas.
"First time Tom Ford has ever let me down," the 55-year-old captioned the snaps. "Ripped pants five minutes in to @TheBeachBoys show in Dallas. I think that Jonas kid is behind this! (or my butt is getting bigger -) #Rippers #Beachbums."
First time @TOMFORD has ever let me down. Ripped pants five minutes in to @TheBeachBoys show in Dallas. I think that Jonas kid is behind this! (or my butt is getting bigger --) #Rippers#Beachbumspic.twitter.com/TZRPQrnqWW— John Stamos (@JohnStamos) 21 March 2019
The stage pants are said to have been part of a three-piece suit, worth more than $5,000 (£3,800).
John and Nick have been engaged in a funny prank war for several months, having each other's faces printed on various clothing and household items and posting the pictures online.
Stamos even recently showed off a fake tattoo he had had of Nick's face inked onto his arm.